Fig. 1

a Schematic representation of the siRNAs used against circ-ZNF609 and/or its linear counterpart, ZNF609 mRNA. Ex.2 = Exon 2; BS J. = back-splicing junction. b Cell cycle analysis by flow cytometry (FACS) of human primary myoblasts in growth conditions upon control treatment (si-SCR) or circ-ZNF609 knock-down (si-Circ). N = 2. c Gene Ontology (GO) term enrichment analysis (Biological Process) performed on genes down regulated (white) and up regulated (gray) upon circ-ZNF609 depletion in human primary myoblasts in growth conditions. d–f RNA levels measured by qRT-PCR of circ-ZNF609 (Circ.), ZNF609 (Lin.) and some genes involved in cell cycle (E2F1, CDK1, Cyclin A2, Cyclin B1, Cyclin B2) in si-SCR and either si-Circ (d), or si-Lin (e), or si-Circ + Lin (f), in human primary myoblasts in growth conditions. Data are shown as means ± standard error of technical duplicates. N = 3