Fig. 5

G9a and cyclin D1 bind common regulatory regions of genes in chromatin immunoprecipitation (ChIP)-Seq. a Venn diagram depicting the overlapping intervals shared between cyclin D1 ChIP-Seq and G9a ChIP-Seq. b Gene Ontology (GO) biological function enrichment scores for overlapping terms for 744 genes common between cyclin D1 ChIP-Seq and G9a ChIP-Seq. c–k Three genes (Mdm4, Pttg1, and Myc) were selected from the “oncogene” GO biological term and eight genes were selected from the “neuronal activities” GO term (Supplemental Figs. 4,5). c, f, i Depicted are tag density profiles for cyclin D1 intervals (red) and G9a intervals (blue) with respect to the identified genes. Profiles generated by Integrated Genome Browser are depicted for enriched regions binding G9a and the same region of cyclin D1 ChIP-Seq. Enriched intervals are designated by an * for cyclin D1 and a * for G9a. Tag density profiles are not drawn to scale (c, f, i). d, g, j Individual ChIP-qPCR analysis of target genes identified in ChIP-Seq. FLAG (FLAG-cyclin D1) ChIP-qPCR analysis of target genes in cyclin D1^−/− plus GFP vector vs cyclin D1^−/− plus cyclin D1^WT rescue mouse embryonic fibroblasts (MEFs), and e, h, k G9a ChIP-qPCR of the same target genes in G9a^−/− plus vector vs G9a^−/− plus G9a^WT rescued MEFs. H3K9me2 ChIP-qPCR is conducted in each cell type with IgG as control. Data are shown as mean ± SEM for ChIP-qPCR of FLAG (FLAG-cyclin D1) and H3K9me2 for target genes identified in ChIP-Seq. Significant difference are shown as **P < 0.01 or *P < 0.05 for cyclin D1^−/− plus GFP vector vs cyclin D1^−/− plus cyclin D1^WT (d, g, j). Mean ± SEM is shown for ChIP-qPCR of G9a and H3K9me2 for Mm44, Pttg1, and c-Myc genes. Significant difference is shown as **P < 0.01 or *P < 0.05 for G9a^−/− plus vector vs G9a^−/− plus G9a^WT (e, h, k)