Fig. 6 | Oncogene

Fig. 6

From: Cyclin D1 integrates G9a-mediated histone methylation

Fig. 6

G9a and cyclin D1 bind common regulatory regions of genes in chromatin immunoprecipitation (ChIP)-Seq in MCF-7 cells. a, b ChIP-qPCR analysis of target genes Pttg1 and c-Myc in MCF-7 cells for G9a and cyclin D1 binding. c Western blot for cyclin D1 in cyclin D1 siRNA-treated MCF-7 cells confirmed decrease of cyclin D1 abundance. d–f Abundance of mRNA for Mdm4 (d), Pttg1 (e), and c-Myc (f) in MCF-7 with cyclin D1 siRNA or control is shown. Data of qPCR is mean ± SEM for n = 3. Eth ethanol, E2 estradiol. g–i ChIP-qPCR analysis of target genes Pttg1 and c-Myc for G9a binding in cyclin D1 rescue cyclin D1−/− mouse embryonic fibroblasts

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