Fig. 2 | Oncogene

Fig. 2

From: LPLUNC1 stabilises PHB1 by counteracting TRIM21-mediated ubiquitination to inhibit NF-κB activity in nasopharyngeal carcinoma

Fig. 2

LPLUNC1 promoted PHB1 ubiquitination. a Co-IP analysis of interaction of LPLUNC1 and PHB1 in CNE1 and HNE1 cells. Normal mouse IgG was used as negative control. b PHB1 and LPLUNC1 expression were detected by immunofluorescence staining (Scale Bar, 10 μm). c Levels of PHB1 proteins in CNE1 and HNE1 cells with or without LPLUNC1 expression following 10 μg/ml CHX treatment for indicated times. d CNE1 and HNE1 cells were co-transfected with Flag-tagged PHB1, myc-tagged LPLUNC1 and HA-tagged ubiqutins. Cells treated with MG132 for 24 h, lysates of CNE1 and HNE1 cells were immunoprecipitated with Flag antibody and analysed by immunoblotting with anti-His antibody to examine ubiquitination of PHB1. Cell lysates were subjected to western blotting with an anti-LPLUNC1 or anti-PHB1 antibody (bottom) in whole-cell lysate. Data shown are representative images each group from three separate experiments

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