Fig. 6
a LPLUNC1 inhibited cell proliferation and promoted apoptosis by upregulating PHB1 expression in CNE1 and HNE1 cells. b CCk-8 assays of cells transfected with LPLUNC1 and control vector, LPLUNC1 and shPHB1 in combination. c Colony-forming assay images (left panel) and quantification of colony number/inoculated number (right panel). d Cell-cycle analysis by flow cytometry. e Annexin V-FITC and PI double staining analysis of cell apoptosis by flow cytometry. f Expression of apoptosis marker Bcl-2 and cell cycle markers cyclinD1 and CDK4 were determined by western blot. α-Tubulin served as an internal control. g Images of CNE1 xenograft model in nude mice. h Tumour growth curve at indicated time. i, j Tumour images and weight quantification of tumours (k) IHC for quantification of proliferation marker Ki-67 and image apoptosis marker Bcl-2 for quantification of apoptosis as well as cell cycle markers Cyclin D1 and CDK4. Scale bar is 50 μm. Data shown are representative images or expressed as the mean ± s.d. of each group from three separate experiments (in vitro) or one separate experiment (in vivo). (*P < 0.05, **P < 0.01 vs. controls, Student’s t-test or two-way ANOVA)
