Fig. 7
LPLUNC1 inhibited LPS-induced NF-κB activation through upregulation of PHB1 in NPC cells. a After LPS (1 µg/ml) treatment for 1 h, NF-κB reporter activity following PHB1 over-expression in CNE1 and HNE1 cells. b immunofluorescence microscopy detection of p65 (red) and nuclei (blue) of CNE1 and HNE1 cells. c Western blotting analysis of p65 in nuclear and cytoplasmic fractions following PHB1 over-expression in CNE1 and HNE1 cells. d Western blotting analysis of NF-κB signal pathway following PHB1 over-expression in CNE1 and HNE1 cells. e Tumours in nude mice were analysed per group using immunohistochemistry for molecules mentioned above. Scale bar is 20 μm. Data shown are representative images or expressed as the mean±s.d. of each group from three separate experiments. (*P < 0.05, **P < 0.01 vs. controls, two-way ANOVA)
