Fig. 2 | Oncogene

Fig. 2

From: Impaired microRNA processing by DICER1 downregulation endows thyroid cancer with increased aggressiveness

Fig. 2

miR-146b inhibition impairs established human thyroid tumor growth. a–c Tumor samples taken from mice treated with a control or an miR-146b inhibitor (Anti-146b) administered intratumorally to tumor xenografts generated as previously described [22]. Tumors were analyzed for DICER1 expression by a qPCR, b immunoblotting, and c immunohistochemistry. Actin was used as a loading control. d Cal62-luc cells were injected into the right thyroid lobe and an orthotopic thyroid tumor was generated. Then, a synthetic miR-146b inhibitor (Anti-146b) or a negative control was administered systemically via the retro-orbital vein. Left: endpoint (day 21) bioluminescent signal of the treated tumors. Right: tumor radiance quantification at the indicated time points in mice from treatment onset with the miRNA inhibitor (blue) or the negative control (green). e Representative immunohistochemistry with an anti-DICER1 antibody in orthotopic tumors. Values represent mean ± SEM. *p < 0.05

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