Fig. 5
Downregulated miR-30a and miR-100 in thyroid cancer block DICER1-silencing-induced effects. Cal62 cells were silenced for DICER1 (siDICER1) and co-transfected with miR-30a or miR-100 plasmids. Control cells were transfected with siControl and the empty DICER1 vector. a Representative images of cells transfected with siControl, siDICER1, siDICER1 + miR-30a, and siDICER + miR-100 at 48 h post-transfection and stained with crystal violet. b BrdU incorporation. c Representative images from a wound healing assay 0, 14, and 24 h after scratching in the above-mentioned conditions. d Quantification of invasion capacity was analyzed in Matrigel-coated Transwells. Left: representative images of the lower chamber (invading cells). Right: cell invasion relative to siControl cells. Values represent mean ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001
