Fig. 2 | Oncogene

Fig. 2

From: Epigenetic reprogramming of primary pancreatic cancer cells counteracts their in vivo tumourigenicity

Fig. 2

Characterization of reprogrammed fibroblasts and PDAC cells. a Expression of pluripotency markers in parental and reprogrammed cells by real-time PCR. b Immunofluorescence staining of pluripotency markers NANOG and OCT4 in the parental and reprogrammed HDF cells. DAPI was used for nuclear counterstaining; scale bar: 50 μm. c Alkaline phosphatase-positive colonies from reprogrammed HDF cells generated by the episomal vectors method

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