Fig. 6 | Oncogene

Fig. 6

From: HDAC7 regulates histone 3 lysine 27 acetylation and transcriptional activity at super-enhancer-associated genes in breast cancer stem cells

Fig. 6

Long-term HDAC7 knockdown decreases total H3K27ac in stem-like BrCa cells. a In CSC-like BPLER2 cells, selective long-term knockdown of HDAC3 with siH3 resulted in significant increase in total H3K27ac levels (3.3-fold) indicating a major role exerted by HDAC3 in maintaining total H3K27ac homeostasis. Extensive HDAC1/3 co-depletion also increased total H3K27ac levels (2.5-fold), all effects that recapitulate the MS-275 effect on stem-like BrCa cells. b In CSC-like BPLER2 cells, serial transfection with HDAC7-specific siRNAs caused a substantial decrease in total H3K27ac levels (10.0-fold). HMLER2 cells did not show substantial reduction in total H3K27ac levels following long-term HDAC1 or HDAC7 depletion. The H3K27ac levels were normalized towards total H3 levels

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