Fig. 2 | Oncogene

Fig. 2

From: Genome-wide mapping of DNA-binding sites identifies stemness-related genes as directly repressed targets of SNAIL1 in colorectal cancer cells

Fig. 2

Significant overlap of genomic-binding sites for Snail1-HA and the intestinal stem cell transcription factors ASCL2 and TCF7L2. a Overlap between genes associated with Snail1-HA ChIP-seq peaks in LS174T cells and components of the intestinal stem cell signature. b Census of regions exhibiting single occurrence, pairwise, and triple colocalization of Snail1-HA, ASCL2, and TCF7L2 ChIP-seq peaks as indicated by dots and vertical connector lines. For each factor, the total number of ChIP-seq peaks found in LS174T cells is shown in parentheses. Information for ASCL2 and TCF7L2 was derived from previously published data sets [29, 77]. Table on the right shows p-values for region-specific colocalization of Snail1-HA, ASCL2, and TCF7L2-binding events. c Genome browser views of RefSeq gene loci depicting the location of ChIP-seq peaks for Snail1-HA, ASCL2, and TCF7L2, and sequence conservation across 100 species (based on hg19 data). The central part shows a blowup of the region around 85 kb downstream of the MYB transcriptional start site (TSS). ChIP-seq peak regions for Snail1-HA, ASCL2, and TCF7L2 are marked by black bars. Regions where Snail1-HA, ASCL2, and TCF7L2 ChIP-seq peaks coincide, are highlighted by red framing

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