Fig. 3

Expression of SNAIL1 and MYB is anticorrelated in colorectal and breast cancer. a Pairwise-correlation analyses of the expression of epithelial and mesenchymal marker genes in 443 colorectal tumor samples (GSE39582) and 466 breast tumor samples (TCGA). The red/blue color shading indicates the Pearson correlation coefficients as shown by the color bar. b, c qRT-PCR and western Blot analyses of MYB, SNAIL1, and SLUG expression in a cohort of CRC and two BRCA cell lines. Transcript levels are depicted as expression relative to GAPDH (rel. expr.). Shown are the mean and SEM; n = 3. GSK3β immunodetection was used as a loading control. MW molecular weight. d qRT-PCR analyses of Snail1-HA and MYB expression in LS174T and HT29 cells stably transduced with Dox-inducible retroviral control (vector) and Snail1-HA expression constructs. Cells were treated with 0.1 μg ml⁻¹ Dox (LS174T) and 1 μg ml⁻¹ Dox (HT29) for the indicated time periods. GAPDH was used for normalization and calculation of relative expression levels (rel. expr.) Shown are the mean and SEM; n = 3. e Western Blot analyses of Snail1-HA and MYB expression in LS174T and HT29 cells stably transduced with Dox-inducible retroviral control (vector) and Snail1-HA expression constructs. Cells were treated with 0.1 μg ml⁻¹ Dox (LS174T) and 1 μg ml⁻¹ Dox (HT29) for the indicated time periods. Detection of RNA polymerase II (POL II) and α-TUBULIN (TUBULIN) served as a loading control. MW: molecular weight