Fig. 2 | Oncogene

Fig. 2

From: Therapeutic anti-cancer activity of antibodies targeting sulfhydryl bond constrained epitopes on unglycosylated RON receptor tyrosine kinase

Fig. 2

RON antibodies stain RON in immunohistochemistry and recognizes endogenous RON in different cancer lines. a Immunohistochemistry staining of RON on HT29 xenograft tumours by hybridoma supernatants. Tumours from HT29 xenograft mice were harvested and embedded in paraffin blocks for immunohistochemistry staining. Analyses showed staining of mainly the cytoplasm and some nuclei. b Western blot analysis of six cancer lines expressing endogenous RON including HCT116, HaCaT, HT29, MKN45, WiDr and T47D was performed using 3G4 and 6E6. H1299 and C6 lines were used as non RON expressing negative controls. Pro-RON and alpha-RON bands were detected in all positive cell lines. c Confocal images showing immunofluorescence staining of HCT116 cells and T47D cells using RON antibodies. Cells were stained with DAPI (blue) for nuclei visualisation and antibodies (green) using anti-mouse/rabbit Ig secondary antibody conjugated with Alexa Fluor 488. Commercial anti-RON rabbit polyclonal antibody C-20 was used as a positive control to visualise RON staining. Polyclonal mouse IgG was used as a negative control. Scale bar: 50 µm

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