Fig. 4

CBX has no effect on normal BM CD34⁺ cells. a CBX treatment (150 µM, 48 h) showed no effect on BM CD34⁺ cell numbers determined by trypan bleu exclusion assay. The results are normalized to untreated cells (n = 5). b CBX treatment did not reduce the colony-forming capacity of CD34⁺ progenitors. Results represent the number of forming colonies (n = 3). c CBX treatment (150 µM, 48 h) did not induce BM CD34⁺ cell apoptosis nor necrosis. The results were obtained using annexin V-FITC and 7AAD staining (n = 5). Results are reported as mean ± SEM. ns nonsignificant. d CBX treatment (150 µM, 48 h) did not modify the frequency of primitive progenitors (CAFC day 35), quantified by limiting dilution assay (n = 3). ns nonsignificant