Fig. 5
CBX reduces AML cell metabolic activity. The oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were concomitantly analyzed by the Seahorse XFe96 Bioanalyzer in three AML cell lines representative of the three groups of response to Ara-C, exposed or not to CBX (150 µM, 48 h). a Representative experiments. The sequential injections were: (1) glucose 10 mM, (2) oligomycin 1 μM, (3) DNP 100 μM, and (4) a mix of rotenone 0.5 μM and antimycin A 0.5 μM. b THP-1, KG1a, and HL-60 cells showed a concomitant decrease in their OCR and ECAR values after exposure to CBX (n = 3). c The effect of CBX on the ability to metabolize 367 substrates was measured by OmniLog® analyzer, revealing a decrease of metabolism capacity of AML cells treated with CBX. The major metabolic modifications induced by CBX concerned the metabolism of glucose, mannose, maltotriose, maltose, glycogen, and dextrin. Heatmap shows data with AUC > 150 in at least one condition. The area under the curve (AUC) of each substrate consumption was measured using the OmniLog® software and normalized in R using opm package to the control conditions, and all AUC values are presented as heatmap
