Fig. 1

Overexpression of miR-200a in human BCs and BBN-treated mouse invasive BCs, and its specific promotion of cell invasion in human invasive BC cell lines. a, b miR-200a expression levels analyzed by real-time PCR in human and mouse BC tissues. c miR-200a expression was analyzed by real-time PCR in UROtsa, T24T, and UMUC3 cell lines. d–i miR-200a expression analyzed by real-time PCR in T24T(Vector) vs. T24T (miR-200a inhibitor) (d) and UMUC3(Vector) vs. UMUC3(miR-200a inhibitor) (g) cells. Invasive abilities of T24T (Vector) vs. T24T (miR-200a inhibitor) (e, f) and UMUC3(Vector) vs. UMUC3(miR-200a inhibitor) (h, i) were analyzed in Invasion Chambers. j–o miR-200a expression was analyzed by real-time PCR in T24T (Vector) vs. T24T (miR-200a) (j) and UMUC3(Vector) vs. UMUC3(miR-200a inhibitor) (m) cells. The invasive abilities of T24T(Vector) vs. T24T(miR-200a) cells and UMUC3(Vector) vs. UMUC3(miR-200a) cells were determined in invasion chambers; and the results were presented as relative invasion in the two paired transfectants, respectively (k, l, n, o). Bars represent means ± SD of three independent experiments. *p < 0.01