Fig. 2: Depletion of FGFR2 in GC cells exhibits an antitumor effect. | Oncogene

Fig. 2: Depletion of FGFR2 in GC cells exhibits an antitumor effect.

From: FGF18–FGFR2 signaling triggers the activation of c-Jun–YAP1 axis to promote carcinogenesis in a subgroup of gastric cancer patients and indicates translational potential

Fig. 2

a The mRNA expression of FGFR2 in AGS, MKN28, and MGC-803 cells after transfection with siFGFR2s (**p < 0.001). b Knockdown of FGFR2 suppressed cell proliferation of the cancer cells (**p < 0.001). c siRNA-mediated knockdown of FGFR2 inhibited monolayer colony formation ability of GC cells (*p < 0.05; **p < 0.001). d Knocking down FGFR2 decreased cell invasion ability (**p < 0.001) (Scale bar, 50 μm). e siFGFR2 induced late and early apoptosis which was manifested by flow cytometry analysis (**p < 0.001). f Cell cycle distribution of siFGFR2 transfectants and siScramble control group by flow cytometry analysis. g Western blot analysis of MARK signaling, cell cycle regulators, and apoptosis biomarker cleaved PARP after FGFR2 knockdown. h The antitumor efficiency of 5-FU after FGFR2 knockdown in AGS and MKN28 was demonstrated by IC50. All experiments were performed in triplicate.

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