Fig. 1: FOXO3a inhibits the invasion and metastasis of breast cancer cells.
From: FOXO3a-driven miRNA signatures suppresses VEGF-A/NRP1 signaling and breast cancer metastasis
A The invasion ability of several breast cancer cell lines was analyzed by the transwell assay. Protein expression of FOXO3a in several breast cancer cell lines was analyzed by Western blot (below). B MDA-MB-231 and BT549 cells were transfected with LV-FOXO3a or LV-Control, and the expression of FOXO3a was analyzed by Western blot. The invasion and migration ability of the cells were determined by the transwell assay (C) and wound-healing assay (D). **P < 0.01. E The expression of EMT markers was analyzed by Western blot. F MDA-MB-231 cells transfected with LV-FOXO3a or LV-control were injected into the tail veins of nude mice and followed over 6 weeks. Representative H&E staining of lung sections is shown. G The numbers of lung micrometastases per section were counted and analyzed.
