Fig. 2: FOXO3a inhibits VEGF-A/NRP1 signaling in breast cancer cells.
From: FOXO3a-driven miRNA signatures suppresses VEGF-A/NRP1 signaling and breast cancer metastasis
A–C MDA-MB-231 and BT549 cells were transfected with LV-FOXO3a or LV-Control; A the expression of VEGF-A, VEGFR-1, VEGFR-2, and NRP1 was analyzed by Western blot; B the expression of VEGF-A, and NRP1 was analyzed by qRT-PCR; C secreted VEGF was measured by ELISA. **P < 0.01. D Photomicrographs showing representative IHC staining results for FOXO3a, VEGF-A, and NRP1 from TMA containing independent primary breast tumor samples. E FOXO3a, VEGF-A, and NRP1 expression in breast cancer tissue sections was quantitatively scored according to the percentage of positive cells and staining intensity as described in “Materials and methods.” Scatter plots showing an inverse correlation between the expression of FOXO3a and VEGF-A or NRP1 and a positive correlation between VEGF-A and NRP1. F Kaplan–Meier survival curves showing the association of FOXO3a, VEGF-A, and NRP1 expression and overall survival in patients with breast cancer.
