Fig. 5: Mechanism dissection how TANAR modulates TWIST1 expression: via stabilizing TWIST1 mRNA. | Oncogene

Fig. 5: Mechanism dissection how TANAR modulates TWIST1 expression: via stabilizing TWIST1 mRNA.

From: Androgen receptor promotes renal cell carcinoma (RCC) vasculogenic mimicry (VM) via altering TWIST1 nonsense-mediated decay through lncRNA-TANAR

Fig. 5

a 786O cells transfected with oeAR pLKO, pWPI pLKO, or oeAR shTANAR were treated with 2 μg/ml actinomycin D for designed times. Real-time RT-PCR was performed to examine the mRNA stability of TWIST1. b Sketch map showed the construct of wild-type TANAR and mutant TANAR. c Western blot assay showed the influence of ectopic wild-type (WT) and mutant (MT) TANAR on TWIST1 protein levels in 786O cells. d RT-PCR showed the influence of ectopic WT and Mut TANAR on TWIST1 mRNA level in 786O cells. e SW839 cells expressing shAR pWPI, shAR oeTANAR WT, shAR oeTANAR MT, or pLKO pWPI, were treated with 2 μg/ml actinomycin D for the designed periods of time. Real-time RT-PCR was performed to examine the mRNA stability of TWIST1. f Co-localization of TANAR (green, upper right) and TWIST1 mRNA (red, lower left) signals using RNA FISH. Nuclei were stained with DAPI (upper left lane). The lower right frame indicates the co-localized region as calculated by Image J colocalization analyzer software. The data are presented as means ± S.D. *p < 0.05, **p < 0.01, and ns = not significant, compared with control.

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