Fig. 3: NONO interacts with PRMT1 in CRC cells.
From: PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer
a Myc-NONO and Flag-PRMT1 interact in HEK 293T cells. pCDH-CMV-Myc-NONO and pCDH-CMV-Flag-PRMT1 were cotransfected into HEK 293T cells for 24 h before coIP analysis. b NONO interacts with PRMT1 in situ. CoIP was performed in KM12 and HCT8 cells using anti-NONO and -PRMT1 antibodies. Arrows indicate NONO or PRMT1, and circles indicate the heavy chain. c NONO and PRMT1 colocalize in the nucleus. Immunofluorescence analysis was performed using anti-NONO and -PRMT1 antibodies in KM12 and HCT8 cells (right panel). NONO and PRMT1 colocalization was analyzed using ZEN v3.0 software (left panel). Scale bar, 10 μm. d NONO interacts with PRMT1. Duolink PLA was performed with indicated antibodies. Blank, no primary antibody added in Duolink PLA. Scale bar, 10 μm. e NONO binds to the catalytic domain of PRMT1. NONO truncations are illustrated schematically at the top. HEK 293T cells were cotransfected with Myc-NONO and Flag-PRMT1 truncations for 24 h before CoIP analysis. aa amino acid, CD catalytic domain, POD post domain, PRD pre domain.
