Fig. 5: LncRNA KB-1980E6.3 increases c-Myc levels by stabilizing its mRNA. | Oncogene

Fig. 5: LncRNA KB-1980E6.3 increases c-Myc levels by stabilizing its mRNA.

From: A novel hypoxic long noncoding RNA KB-1980E6.3 maintains breast cancer stem cell stemness via interacting with IGF2BP1 to facilitate c-Myc mRNA stability

Fig. 5

a, b Luciferase reporter assay showing the transcription activity of c-Myc in lncRNA KB-1980E6.3 knockdown and the control breast cancer cells under normoxia or hypoxia conditions. STAT3 knockdown was used as a positive control. c, d qRT-PCR was used to determine c-Myc mRNA levels in lncRNA KB-1980E6.3 knockdown and the control breast cancer cells treated with Actinomycin D for the indicated time under hypoxia conditions. e, f qRT-PCR was used to determine c-Myc mRNA levels in lncRNA KB-1980E6.3 overexpressing and the control breast cancer cells treated with Actinomycin D for the indicated time under normoxia conditions. Data are shown as the mean ± SD of three independent experiments. (ns no significance; *P < 0.05; **P < 0.01).

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