Fig. 6: LncRNA KB-1980E6.3 increases the stability of c-Myc mRNA through binding with IGF2BP1. | Oncogene

Fig. 6: LncRNA KB-1980E6.3 increases the stability of c-Myc mRNA through binding with IGF2BP1.

From: A novel hypoxic long noncoding RNA KB-1980E6.3 maintains breast cancer stem cell stemness via interacting with IGF2BP1 to facilitate c-Myc mRNA stability

Fig. 6

a The probability of the interaction between lncRNA KB-1980E6.3 and IGF2BP1 was predicted by RNA-Protein interaction prediction (RPISeq). b RIP assay was performed using cell lysates from normoxic or hypoxic breast cancer cells using anti-IGF2BP1 antibody. IgG served as a negative control. The lncRNA KB-1980E6.3 enrichment in the RIP precipitates was analyzed by qRT-PCR. GAPDH mRNA served as a negative control. c The enriched c-Myc in the RIP precipitates derived from normoxic and hypoxic BT549 and Hs578T cells was analyzed by qRT-PCR. GAPDH mRNA served as a negative control. d Lysates from hypoxic BT549 and Hs578T cells were subjected to RNA-pulldown with biotin-labeled lncRNA KB-1980E6.3 sense or antisense probe, followed by western blotting with anti-IGF2BP1 antibody. GAPDH was used as a negative control. e Top, schematic diagrams of lncRNA KB-1980E6.3 full-length and truncated fragments. Bottom, the interaction between lncRNA KB-1980E6.3 truncates and IGF2BP1 in hypoxic BT549 cells was examined by western blotting. f Top, diagrams of full-length and truncated fragments of IGF2BP1. Bottom, the immunoprecipitation efficiency of HA-tagged full-length or truncated IGF2BP1 in RIP assays (left panel). RIP-qPCR was used to identify the lncRNA KB-1980E6.3 binding domain in IGF2BP1 using full-length or truncated IGF2BP1 protein (right panel). Data are shown as mean ± SD of three independent experiments (**P < 0.01; ***P < 0.001).

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