Fig. 7: LncRNA KB-1980E6.3 increases the stability of c-Myc mRNA through recruitment of IGF2BP1 to bind with m6A-modified c-Myc CRD mRNA. | Oncogene

Fig. 7: LncRNA KB-1980E6.3 increases the stability of c-Myc mRNA through recruitment of IGF2BP1 to bind with m6A-modified c-Myc CRD mRNA.

From: A novel hypoxic long noncoding RNA KB-1980E6.3 maintains breast cancer stem cell stemness via interacting with IGF2BP1 to facilitate c-Myc mRNA stability

Fig. 7

a, b The plasmid of c-Myc wild type CRD (WT) or mutated CRD (MUT) was co-transfected with or without siIGF2BP1 into endogenous c-Myc knockdown BT549 cells or c-Myc and lncRNA KB-1980E6.3 double knockdown BT549 cells, respectively. The indicated BT549 lysates were incubated with either sense or antisense biotin-labeled probe against lncRNA KB-1980E6.3 for the RNA pull-down assay. Western blotting was used to detect the IGF2BP1 protein (a), and qRT-PCR was used to detect lncRNA KB-1980E6.3 (b) in the pull-down precipitates. c qRT-PCR was used to determine the enrichment of c-Myc CRD mRNA by lncRNA KB-1980E6.3-recruited IGF2BP1 in the pull-down precipitates. d Gene-specific m6A qPCR assays were performed in cellular lysates from the indicated engineered cells described in (a) to detect the m6A-modified c-Myc CRD mRNA levels. e The indicated engineered BT549 cells described in (a) were treated with Actinomycin D for the indicated times, and c-Myc mRNA levels were measured by qRT-PCR. f, g c-Myc mRNA and protein levels were examined in the engineered cells described in (a) by qRT-PCR and western blotting. Data are shown as mean ± SD of three independent experiments (**P < 0.01; ***P < 0.001).

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