Fig. 2: Assessment of Bmi1-dependency of deregulated genes.
From: Polycomb-mediated repression of EphrinA5 promotes growth and invasion of glioblastoma
a Western blot (left) and quantification (right) showing effective Bmi1 silencing in shBmi1 mGIC compared with the control shScr (n = 4). b qPCR analysis of Bmi1-dependency of candidate gene expression (n = 3). c Visualisation of the EfnA5 locus, centred on the TSS, shows a differential H3K27me3 distribution (green) and expression (blue) between mGIC and the non-neoplastic NSC and NSC Bmi1Over. d Schematic representation of the EfnA5 region where PS 3.2 and PS 3.5 primer pairs allign (top), and quantification of qChIP assay (bottom) confirms that the H3K27me3 levels depend on Bmi1 expression in mGIC. Immunoprecipitated chromatin (mock) was used as negative control (n = 6). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; error bars represent ±SEM.
