Fig. 5: Inhibition of IP3R by xestospongin C (XeC) or MCU by ruthenium red (RuR) reverses the opening of mPTP in Cdk5−/− MEFs.
From: mPTP opening caused by Cdk5 loss is due to increased mitochondrial Ca2+ uptake
a Wt and Cdk5−/− MEFs pretreated with or without XeC (3 µM) or RuR (3 µM) were loaded with calcein-AM, then treated with or without CoCl2. Cell were then analysed by flow cytometry. Cells treated with the potent mPTP desensitizers, cyclosporin A (CsA, 1 µM) or sanglifehrin A (SFA, 2 µM), were used as positive controls. b Quantitative analysis of the relative calcein fluorescence in wt and Cdk5−/− MEFs in the presence or absence of the treatments indicated above. Untreated cells and cells treated with ionomycin served as controls. Values are means ± SEM from three (n = 3) independent experiments. *p < 0.05 by unpaired Student’s t test.
