Fig. 1: Impact of vimentin on TF expression and coagulant properties of EMT+ tumor cells. | Oncogene

Fig. 1: Impact of vimentin on TF expression and coagulant properties of EMT+ tumor cells.

From: Vimentin prevents a miR-dependent negative regulation of tissue factor mRNA during epithelial–mesenchymal transitions and facilitates early metastasis

Fig. 1

a Western blotting analyses of TF and GAPDH in MDA-MB-231, in MDA-MB-468 cells induced to EMT by EGF, in PMC42-LA cells induced to EMT by EGF, in human lung tumor A549 cells induced to EMT by TGF-β1, and human skin fibroblasts, and transfected with two nontargeting siRNA (Ctrl Si1 or Ctrl Si2) or two siRNA against vimentin (Vim Si1 or Vim Si2). The results of corresponding in vitro coagulation assays, performed by incubating whole blood of healthy donors with cells transfected, are given underneath the western blots. b FACS analyses of surface TF expression in cells treated as in a.

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