Fig. 2: Impact of vimentin silencing on metastatic colonization. | Oncogene

Fig. 2: Impact of vimentin silencing on metastatic colonization.

From: Vimentin prevents a miR-dependent negative regulation of tissue factor mRNA during epithelial–mesenchymal transitions and facilitates early metastasis

Fig. 2

RT-nested qPCR for human GAPDH performed on total RNA extracted from lungs of BALB/c mice injected intravenously with EGF-treated MDA-MB-468 cells (a) (n = 11) or MDA-MB-231 (b) (n = 5) transfected with a non-targeting control siRNA (Ctrl Si1) or a siRNA against vimentin (Vim Si1) and collected 24 h after injection. c RT-nested qPCR for human GAPDH performed on total RNA extracted from lungs of SCID mice injected intravenously with MDA-MB-231 silenced for vimentin or not and sacrificed 3 weeks after injections (n = 8). Double immunofluorescence against human Ki67 (red) and mouse VWF (green) performed on lung sections. Nuclei were labeled with DAPI (blue).

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