Fig. 1: ADAR1 knockdown reduces thyroid cancer cells proliferation and 3D growth. TPC1, Cal62, and 8505c cell lines were transfected with two different siRNAs against ADAR1 (siADAR1 #1 and siADAR1 #2) or a control siRNA (siControl). | Oncogene

Fig. 1: ADAR1 knockdown reduces thyroid cancer cells proliferation and 3D growth. TPC1, Cal62, and 8505c cell lines were transfected with two different siRNAs against ADAR1 (siADAR1 #1 and siADAR1 #2) or a control siRNA (siControl).

From: ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity

Fig. 1

a MTT assay at the indicated time points. b Upper panel: representative images of crystal violet-stained colonies. Bottom panel: quantification of crystal violet absorbance. c Immunoblot of ADAR1 and proliferating cell nuclear antigen (PCNA) at 72 and 96 h after transfection. GAPDH was used as a loading control. d 3D cell culture. Values represent mean ± SD (n = 3). Asterisks represents significant difference between siADAR1 #1 and siControl, and Hash represents significant difference between siADAR #2 the siControl. */#p < 0.05; **/##p < 0.01; ***/###p < 0.001.

Back to article page