Fig. 2: ADAR1 knockdown reduces thyroid cells invasion, migration in vitro and xenograft tumor growth in vivo. | Oncogene

Fig. 2: ADAR1 knockdown reduces thyroid cells invasion, migration in vitro and xenograft tumor growth in vivo.

From: ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity

Fig. 2

TPC1, Cal62, and 8505c cell lines were transfected with two different siRNAs against ADAR1 (siADAR1 #1 and siADAR1 #2) or a control siRNA (siControl). a Quantification of cell invasion. Upper panel: representative images of the lower chamber (invading cells). Bottom panel: cell invasion relative to siControl cells. b Quantification from a wound-healing assay at the indicated time points after scratching. c, d Xenograft tumors were generated by subcutaneous injection with Cal62-Luc cells transfected previously with siControl (n = 8), siADAR1 #1 (n = 7), or siADAR1 #2 (n = 7). c Endpoint (day 18) bioluminescent signal of the generated tumors. d Tumor radiance quantification at the indicated time points. Values represent mean ± SD (n = 3). ***p < 0.001 for the in vitro techniques (A and B), and mean ± SEM. */#p < 0.05; **/##p < 0.01 for the in vivo experiment (d).

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