Fig. 3: The ADAR1-dependent editing in miR-200b, increased in thyroid cancer patients, decreasing its binding to ZEB1 3′UTR. | Oncogene

Fig. 3: The ADAR1-dependent editing in miR-200b, increased in thyroid cancer patients, decreasing its binding to ZEB1 3′UTR.

From: ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity

Fig. 3

a Immunoblot for ADAR1 and ZEB1 72 and 92 h after TPC1 and Cal62 siControl, siADAR1 #1 or siADAR1 #2 transfection. GAPDH was used as a loading control. b Left: relative miR-200b edited (Edit)/wild-type (WT) miRNA levels in six PTC patients (contralateral and normal thyroid tissue). Right: total average of miR-200b Edit/WT miRNA relative levels. c miR-200b Edit/WT miRNA relative levels in TPC1 and Cal62 cells transfected with the siRNAs siControl, siADAR1 #1 or siADAR1 #2. d Renilla luciferase reporter activity relative to Firefly luciferase was evaluated 72 h after transfection of ZEB1 3′UTR or the same construction with mutated predicted miR-200b-3p binding sites (ZEB1 3′UTR miR-200b mut) in Cal62 cells co-transfected with the siRNAs siControl, siADAR1 #1 or siADAR1 #2. e Renilla luciferase reporter activity relative to Firefly luciferase was evaluated 72 h after transfection of ZEB1 3′UTR or the same construction with mutated predicted miR-200b-3p binding sites (ZEB1 3′UTR miR-200b mut) in TPC1 and Cal62 cells co-transfected with the WT miR-200b or edited (edit) miR-200b mimics or a negative control (Neg.Control). Values represent mean ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001.

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