Fig. 4: Editing of miR-200b impairs its ability to inhibit ZEB1, modulating the thyroid cell invasion capacity. | Oncogene

Fig. 4: Editing of miR-200b impairs its ability to inhibit ZEB1, modulating the thyroid cell invasion capacity.

From: ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity

Fig. 4

a Left: representative immunoblot for ADAR1 and ZEB1 72 h after transfection with WT miR-200b or edited (edit) miR-200b mimics or negative control in TPC1 and Cal62 cells. GAPDH was used as a loading control. Right: quantification relative to the negative control transfected cells. b Cal62 cells were co-transfected with an siRNA (siControl or siADAR1) and an miRNA mimic (negative control, WT miR-200b wt or edit miR-200b mimics). Upper panel: representative images of the lower chamber (invading cells). Bottom panel: quantification of invasion relative to siControl—Neg. Control cells. Values represent mean ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001, ns nonsignificant.

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