Fig. 2: siRNA-mediated loss-of-function screen of candidate driver genes in TNBC cells. | Oncogene

Fig. 2: siRNA-mediated loss-of-function screen of candidate driver genes in TNBC cells.

From: Integrative analysis of genomic amplification-dependent expression and loss-of-function screen identifies ASAP1 as a driver gene in triple-negative breast cancer progression

Fig. 2

a Replicate siRNA screens of candidate driver genes in TNBC cell lines BT549 and SUM149T. siRNA silencing effect of candidate gene on cell proliferation was assessed 96 h after transfection and presented with sulforhodamine B (SRB) colorimetric raw values. b Normalized percentage of proliferation control by siRNA silencing. The number of genes (black) whose silencing led to >40% proliferative inhibition was indicated in the pie chart, as primary hits. c Ranking and listing of the primary hits significantly controlling proliferation of BT549 and SUM149PT TNBC cell lines. siRNA targeting KIF11, positive functional control; siCtrl, non-targeting siRNA control. siRNA silencing effect on proliferation was relative to siCtrl. Error bars indicate variation of screen replicates. d Overlap primary hits in BT549 and SUM149T TNBC cell lines.

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