Fig. 3: PDGFC secreted from CAFs is required for GIST growth in vitro and in vivo. | Oncogene

Fig. 3: PDGFC secreted from CAFs is required for GIST growth in vitro and in vivo.

From: Cancer-associated fibroblast secretion of PDGFC promotes gastrointestinal stromal tumor growth and metastasis

Fig. 3

a–c Knockdown of PDGFC in GIST-CAFs. PDGFC was silenced by shPDGFC #1 and shPDGFC #2 using lenti-virus conjugated with green fluorescent protein (GFP). The efficiency of knockdown was confirmed by qPCR (a), immunoblotting analysis (b), and ELISA (c). All graphs show mean ± SEM. p values were represented by ANOVA analysis. ***p < 0.001. d Effects of stable PDGFC knockdown of CAF in T1 proliferation. T1 cells were co-cultured with CAFscr or CAFshPDGFC #1–2 for 72 h. The numbers of cells were counted using an Automated Cell Counter. p values were represented by ANOVA analysis. ***p < 0.001. e–g Tumor burden analysis in mice bearing T1, T1 + CAFscr, and T1 + CAFshPDGFC #1–2. T1 cells expressing mCherry were injected subcutaneously into mice (n = 8) alone or with CAFscr or CAFshPDGFC #1–2. Representative in vivo imaging system (IVIS) images (e) showing for all 4 groups after injection for 6 weeks and graphical representative (f) of tumor growth over time as quantified by total photon flux (p/s). Tumor weight (g) of each group was measured after all tumors were harvested. p values were represented by ANOVA analysis. *p < 0.05, **p < 0.01, ***p < 0.001. Representative immunohistochemistry (IHC) images (h) stained for Ki67, a hallmark of cell proliferation, in the tumor sections collected from each group and quantification (i) indicated by Ki67 positive pixel ratio. The intensities of IHC images were analyzed using Aperio ImageScope. p values were represented by ANOVA analysis. ***p < 0.001. Scale bars, 100 µm.

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