Fig. 8: A role for IL-6 and STAT3 in activation of NF-κB transcriptional activity in TKI resistance. | Oncogene

Fig. 8: A role for IL-6 and STAT3 in activation of NF-κB transcriptional activity in TKI resistance.

From: Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B

Fig. 8

A Bar graph shows relative IL6 and TNFa mRNA levels in TKI-sensitive K562S cells versus TKI-resistant K562R cells in the presence and absence of imatinib (1 μM, 24 h). Bar graphs represent the concentration of TNFα (B) or IL-6 (C) produced by K562S versus K562R cells in the presence and absence of imatinib (1 μM, 24 h). D Immunoblot shows total STAT3, phospho-STAT3Y705, total NF-κB, and phospho-NF-κB in K562S and K562R cells upon induction of shRNA targeting STAT3 (shSTAT3) with doxycycline (100 ng/ml, 72 h). E Bar graph represents NF-κB luciferase reporter activity in K562S versus K562R cells upon induction of shSTAT3 with doxycycline (100 ng/ml, 72 h). Error bars represent SEM.

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