Fig. 3: ST6GAL1 K.O. upregulates the cell surface expression of alternative glycan capping motifs. | Oncogene

Fig. 3: ST6GAL1 K.O. upregulates the cell surface expression of alternative glycan capping motifs.

From: ST6Gal1 targets the ectodomain of ErbB2 in a site-specific manner and regulates gastric cancer cell sensitivity to trastuzumab

Fig. 3

Cell surface flow cytometric staining of NCI-N87 WT and ST6GAL1 K.O. ErbB2-positive cells with the (A) Sambucus nigra agglutinin (SNA) recognizing α2,6-linked sialic acid (α2,6NeuAc); B Maackia amurensis lectin II (MAL-II) recognizing α2,3-linked sialic acid (α2,3NeuAc); C CA 19.9 monoclonal antibody (mAb) recognizing sialyl Lewis a (SLea); D Aleuria aurantia lectin (AAL) recognizing α1,6- and α1,3/4-linked fucose (α1,6Fuc and α1,3/4Fuc); E Lotus tetragonolobus lectin (LTL) recognizing α1,2-linked fucose (α1,2Fuc); F Ulex europaeus agglutinin I (UEA-I) recognizing α1,2-linked fucose (α1,2Fuc); Comparisons were made using one-way ANOVA analysis of variance (n = 3; mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001); n.s. nonsignificant, C1 clone 1, C2 clone 2, C3 clone 3.

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