Fig. 4: TTPAL activated PI3K/AKT signaling.
From: TTPAL promotes gastric tumorigenesis by directly targeting NNMT to activate PI3K/AKT signaling
A KEGG pathways enriched by differentially expressed genes affected by TTPAL. B Downstream targets of TTPAL identified by PI3K/AKT signaling pathway PCR array. C Ectopic expression of TTPAL significantly reduced FOXO reporter activity while knockdown of TTPAL significantly suppressed PI3K/AKT activity. D Western blot results showed that TTPAL expression enhanced the expression of PI3K/AKT signaling-related markers, while knockdown of TTPAL showed the opposite effect. E PI3K/AKT signaling inhibitor GDC-0941 treatment abolished the promoting effects of TTPAL on cell proliferation and clonogenicity (BGC823, 0.5 μmol/l and MGC803, 1 μmol/l). F. AKT knockdown also abolished the tumor-promoting function of TTPAL.
