Fig. 4: Linc00284 directly binds to miR-27a and negatively regulates miR-27a expression. | Oncogene

Fig. 4: Linc00284 directly binds to miR-27a and negatively regulates miR-27a expression.

From: Oncogenic long intervening noncoding RNA Linc00284 promotes c-Met expression by sponging miR-27a in colorectal cancer

Fig. 4

A Schematic diagram of the potential binding sites between Linc00284 and miR-27a. B Dual-luciferase reporter assay and C RIP experiment confirmed the interaction between Linc00284 and miR-27a. D Binding of miR-27a and Ago2 determined by RIP assay. E The expression level of miR-27a in Linc00284 knockdown CRC cells. F miR-27a level in HCT116 cells transfected with pcDNA Linc00284 vectors or si Linc00284 for 48 h. G miR-27a expression in SW480 cells transfected with pcDNA Linc00284 or si Linc00284 for 48 h. H miR-27a expression in human CRC tissues was detected by qPCR analysis (N = 73). I Pearson correlation test suggested a negative correlation between Linc00284 and miR-27a expression. J Serum miR-27a level in patients with CRC (N = 73) compared to healthy controls (N = 43). Data are presented as the mean ± S.D. from three independent experiments. Comparisons between two groups were analyzed using two-sided paired t-test. Multiple comparisons were analyzed using one-way analysis of variance (ANOVA), post-hoc least significant difference (LSD) test. *P < 0.05; **P < 0.01; ***P < 0.001; NS not significant difference, sh00284 lentiviral vector-mediated Linc00284 silencing by short hairpin RNA.

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