Fig. 5: Linc00284 suppresses the proliferation, migration and invasion of CRC cells by targeting miR-27a.

A miR-27a expression in normal colonic epithelial cells FHC and CRC cell lines (HCT116 and SW480) was detected by qPCR analysis. B The effect of miR-27a inhibitor on the expression of miR-27a in Linc00284 knockdown CRC cells. Left, HCT116 cells; right, SW480 cells. C The effect of miR-27a inhibitor on CRC cell proliferation was measured by CCK-8 assay. Left, HCT116 cells; right, SW480 cells. D The effect of miR-27a inhibitor on the proliferation of Linc00284 knockdown CRC cells was determined by colony formation assay. E Ki-67 staining was used to test the effect of miR-27a inhibitor on the proliferation of Linc00284 knockdown CRC cells. F The migration ability of CRC cells was measured by wound healing assay. G The invasive ability of CRC cells was monitored by transwell assay. Xenograft tumors model for effect of Linc00284 knockdown alone or in combination with miR-27a (H) mimics/(I) inhibitor treatment on the growth of HCT116 xenografts. Tumor growth was monitored by measurements of tumor diameter using calipers (left); the tumor volume (middle); the expression of Linc00284, miR-27a and c-Met in tumor tissues (right). Data are presented as the mean ± S.D. from three independent experiments. P values were calculated using one-way analysis of variance (ANOVA), post-hoc least significant difference (LSD) test. *P < 0.05; **P < 0.01; ***P < 0.001. sh 00284, lentiviral vector-mediated Linc00284 silencing by short hairpin RNA.