Fig. 6: Linc00284 regulates c-Met expression by adsorbing miR-27a.

A Schematic diagram of the binding sites between miR-27a and 3′UTR of c-Met mRNA. B The luciferase activity was tested after co-transfection with wild type (WT) 3′UTR of c-Met mRNA/mutant 3′UTR of c-Met mRNA and miR-27a mimics for 48 h. RIP assay was used to determine the interaction between (C) miR-27a and (D) c-Met in CRC cells. E The expression of c-Met in 73 paired CRC tissues and adjacent tissues was tested by qPCR analysis. F Pearson correlation analysis showed the correlation between c-Met and miR-27a expression. G The correlation between c-Met and Linc00284 expression. H The effects of Linc00284 knockdown or in combination with miR-27a inhibitor treatment for 48 h on the mRNA and protein expression of c-Met in HCT116 cells. I The effects of Linc00284 overexpression or in combination with miR-27a mimics treatment for 48 h on the mRNA and protein expression of c-Met in HCT116 cells. Data are presented as the mean ± S.D. from three independent experiments. Comparison between two groups were analyzed using two-sided paired student’s t-test. Multiple comparisons were analyzed using one-way analysis of variance (ANOVA), post-hoc least significant difference (LSD) test. **P < 0.01; ***P < 0.001; NS not significant difference, sh00284 lentiviral vector-mediated Linc00284 silencing by short hairpin RNA, OE 00284 overexpression of Linc00284.