Fig. 2: MIR17HG promotes glycolysis in colorectal cancer cells. | Oncogene

Fig. 2: MIR17HG promotes glycolysis in colorectal cancer cells.

From: LncRNA MIR17HG promotes colorectal cancer liver metastasis by mediating a glycolysis-associated positive feedback circuit

Fig. 2

A Expression levels of MIR17HG in eight colorectal cancer cell lines (Caco2, HT29, SW480, SW620, RKO, HCT116, HCT8, and DLD-1) and one normal colon cell line (FHC) were determined by quantitative RT-PCR. B–C The efficiency of MIR17HG overexpression (B) and knockdown (C) in the indicated cells was validated by quantitative RT-PCR. Controls (ex-NC or sh-NC). D Changes in relative glucose consumption, ATP levels and lactate production in SW480 and HT29 cells after MIR17HG overexpression; E Changes in relative glucose consumption, ATP levels and lactate production in SW620 and RKO cells upon MIR17HG knockdown; F, G Changes in ECAR levels in MIR17HG-overexpressing SW480 (F) and HT29 (G) cells. The ECAR after oligomycin treatment indicates glycolytic capacity; H, I Changes in glycolytic capacity upon MIR17HG knockdown in SW620 (H) and RKO (I) cells. *P < 0.05, **P < 0.01, ***P < 0.001.

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