Fig. 2: HECTD2 expression drives the cell cycle in human melanoma cell lines.
From: E3 ubiquitin ligase HECTD2 mediates melanoma progression and immune evasion
a Distribution of genes differentially upregulated in high HECTD2 expressing samples, according to the phase of the cell cycle they are functionally annotated to be involved in. b Expression of HECTD2 (TPM) in the indicated human melanoma cells lines in data from CCLE. c Cell duplication times (left) and dry mass duplication times (right) of parental IGR-1 human melanoma cells and IGR-1 cells overexpressing HECTD2 (IGR-1.HECTD2). Symbols represent the mean values of separate wells (n = 24), with 4 fields of view averaged per well, from three independent experiments. d Representative example of cell perimeter and cell area demarcation using Livecyte segmentation (left) and cell perimeters and cell areas of IGR-1 and IGR-1.HECTD2 cells. Symbols represent individual cells in one of three independent experiments. e Total cell counts (left) and total dry mass (right) in cultures of IGR-1 and IGR-1.HECTD2 cells over time. Plotted are the mean values (±SEM) of the mean of each of the three independent experiments. P values were calculated with Mann–Whitney Rank Sum tests.
