Fig. 1: A SNAI2-PEAK1 axis correlates with disease relapse and co-stains the non-epithelial fibroblastic stroma in HER2-positive breast cancer. | Oncogene

Fig. 1: A SNAI2-PEAK1 axis correlates with disease relapse and co-stains the non-epithelial fibroblastic stroma in HER2-positive breast cancer.

From: A SNAI2-PEAK1-INHBA stromal axis drives progression and lapatinib resistance in HER2-positive breast cancer by supporting subpopulations of tumor cells positive for antiapoptotic and stress signaling markers

Fig. 1

a Kaplan–Meier relapse-free survival (RFS) curves for patients with low or high PEAK1 transcript levels across all breast cancer subtypes (n = 1784). b Kaplan–Meier RFS curves for HER2-positive breast cancer patients with low or high PEAK1 transcript levels (n = 272). c PEAK1 transcript levels in normal breast stroma and breast cancer stroma across all subtypes (n = 6 and 53, respectively). d PEAK1 transcript levels in breast cancer stroma of relapse-free patients and those with disease recurrence across all subtypes (n = 42 and 11, respectively). e Expression correlation analysis of PEAK1 and ETS1, KLF4, SNAI2, FN1, FOXC2 or MYC in patients having mixed or good outcomes (top, n = 45) and patients having poor outcomes (bottom, n = 8). f Representative IHC images for HER2, PEAK1, SNAI2, MYC and FN1 in breast cancer tissue where PEAK1 expression in the stromal compartment is low (top) or high (bottom). g Stromal IHC score correlation and linear regression analyses of PEAK1 and SNAI2, MYC or FN1 across six breast cancer tissue samples (patient #s 1874, 1939, 2428, 1920, 2392 and 3257). h Average stromal IHC scores for PEAK1, SNAI2, MYC and FN1 in three HER2-negative patients and three HER2-positive patients. i–k Violin plots of quantified PEAK1, SNAI2 or PEAK1/SNAI2 stromal expression from IHC/IF data on a 144-breast cancer sample tissue microarray (79 HER2-positive cases). j–m Representative 3D deconvolution widefield microscopy images for PEAK1 and SNAI2 in TB130 (HER2-negative) and TB122 (HER2-positive) breast cancer tissues. n–o Kaplan–Meier relapse-free survival (RFS) curves for patients with low or high SNAI2 in all breast cancer subtypes (n, n = 1784) or HER2-positive breast cancer (o, n = 272). Kaplan–Meier relapse-free survival (RFS) curves for patients with low or high PEAK1 in all SNAI2low or SNAI2high breast cancers (p and r, n = 892) and HER2-positive SNAI2low or SNAI2high breast cancers (q and s, n = 136). *, **, *** and **** indicates p value < 0.05, 0.01, 0.001 and 0.0001, respectively, as determined by a Student’s T test.

Back to article page