Fig. 5: MSC expression of PEAK1 protects neighboring breast cancer cells from lapatinib-induced cytotoxicity. | Oncogene

Fig. 5: MSC expression of PEAK1 protects neighboring breast cancer cells from lapatinib-induced cytotoxicity.

From: A SNAI2-PEAK1-INHBA stromal axis drives progression and lapatinib resistance in HER2-positive breast cancer by supporting subpopulations of tumor cells positive for antiapoptotic and stress signaling markers

Fig. 5

a Schematic of breast cancer cell mono- or breast cancer cell-CAF/MSC co-culture using non-labeled stromal fibroblasts and H2B-eGFP+ BT474 breast cancer cells for downstream analysis of breast cancer cell number and death using the IncuCyte imaging system over 96 h (48 h pre-incubation and 48 h incubation with therapy and EtBr). b–c Endpoint dose-response curves for lapatinib effects on breast cancer cell number (b) or cell death (c) in the indicated breast cancer cell and stromal fibroblast culture combinations. d Quantification of tumor cell number (left) and EtBr uptake (right) at assay endpoint for BT474 cells alone or co-cultured with the shScr or shP1(5) derivatives of C3H10T1/2 cells and treated with vehicle control or the indicated dose of lapatinib. * of **** indicates a p valule of 0.05 or 0.0001, respectively as determined by a Two-Way ANOVA with multiple comparisons post-test.

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