Fig. 2: ZNF545 directly binds to the minimal rDNA promoter region. | Oncogene

Fig. 2: ZNF545 directly binds to the minimal rDNA promoter region.

From: ZNF545 loss promotes ribosome biogenesis and protein translation to initiate colorectal tumorigenesis in mice

Fig. 2

a Schematic representation of pHrD-IRES-Luc and two deletion constructs. b The effect of ZNF545 on rDNA promoter activity from pHrD-IRES-Luc and two deletion constructs assessed by dual-luciferase reporter assay (each group: n = 3). All histogram data represent mean ± SD (two-sided Student’s t test). Compared with control, **P < 0.01; ***P < 0.001. c Schematic representation of six overlapping biotinylated DNA probes spanning the minimal rDNA promoter region for EMSA. d Binding activity of the six biotinylated DNA probes to the nuclear extracts from Flag-tagged ZNF545-expressing and control HCT116 cells measured by EMSA. e EMSA competition and supershift assays of probes 2, 3, and 6. Competition reactions were performed by adding 100-fold unlabeled probes. Supershift reactions were performed by preincubation of nuclear extracts from Flag-tagged ZNF545-expressing HCT116 cells with anti-Flag antibody prior to the addition of the biotinylated DNA probes.

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