Fig. 2: FBP1 overexpression sensitizes ovarian cancer cells to cisplatin. | Oncogene

Fig. 2: FBP1 overexpression sensitizes ovarian cancer cells to cisplatin.

From: FBP1 regulates proliferation, metastasis, and chemoresistance by participating in C-MYC/STAT3 signaling axis in ovarian cancer

Fig. 2

A IF staining of the representative human ovarian cancer organoid lines with PAX8, FBP1, and DAPI (DNA) as indicated. B CCK-8 assays showed the effect of empty vector and FBP1 OE on the chemosensitivity of ovarian cancer cells to the cytotoxic effect of cisplatin. C The percentage of apoptotic cells in indicated group after cisplatin treatment. Cells were stained with annexin V–fluorescein isothiocyanate (FITC) and propidium iodide (PI) to detect cells in early apoptosis (annexin V+ PI–) and late apoptosis (annexin V+ PI+). Representative pictures are shown. D The expression levels of Bcl-2 and BAX in vector control (Vec) and FBP1 overexpressing (FBP1 OE) ovarian cancer cells were examined by western blotting. E Representative images showed the representative number of spheres counted each day over a period of 7 days for A2780 cells stably expressing empty vector (Vec) or FBP1 (FBP1 OE). F, G The percentage of ALDH-positive ovarian cancer cells in the vector and FBP1 OE groups were determined by flow cytometry and statistically analyzed. H Immunoblotting analysis of the apoptosis-associated proteins SOX2, OCT4 and NANOG. Data are shown as the means ± SD. *P < 0.05, **P < 0.01.

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