Fig. 4: FBP1 directly interacts with STATS and inhibits STAT3 expression and phosphorylation in ovarian cancer cells. | Oncogene

Fig. 4: FBP1 directly interacts with STATS and inhibits STAT3 expression and phosphorylation in ovarian cancer cells.

From: FBP1 regulates proliferation, metastasis, and chemoresistance by participating in C-MYC/STAT3 signaling axis in ovarian cancer

Fig. 4

A Interaction between FBP1 and STAT3 detected by co-immunoprecipitation assay. B Interaction between FBP1 and STAT3 by FRET-FLIM upon transient coexpression in A2780 and SKOV3 cells. FE, FRET efficiency. Asterisks indicate a statistically significant difference (**P value < 0.01), according to the Student’s t test. C, D The influence of induction of FBP1 on the distribution and expression of STAT3 and p-STAT3 (Tyr705) protein in the nucleus and cytoplasm. E Representative immunofluorescence staining (×1000) images showing that FBP1 inhibited the expression of STAT3 in the cell nucleus (red). Blue dye (DAPI) indicates the nucleus. F Schematic image of functional domains of STAT3. G Western blot analysis indicated proteins ectopically expressed six regions of STAT3 and seven exons of FBP1. H Co-immunoprecipitation analysis indicated the interaction between STAT3 domains and FBP1 exons. I RT-PCR results of ChIP. The experiments were conducted in triplicate, and a representative experiment is shown.

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