Fig. 5: FBP1 was regulated directly by C-MYC in ovarian cancer cells. | Oncogene

Fig. 5: FBP1 was regulated directly by C-MYC in ovarian cancer cells.

From: FBP1 regulates proliferation, metastasis, and chemoresistance by participating in C-MYC/STAT3 signaling axis in ovarian cancer

Fig. 5

A The correlation between FBP1 mRNA expression level and the degree of methylation. B Enrichment of C-MYC at the promoter of FBP1, figure was download from UCSC Genome Bioinformatics Site (http://genome.ucsc.edu/). C Representative images of methylation-specific PCR results from ovarian cancer patients and healthy women. D Western blotting analysis of FBP1 protein expression in cells with the silencing of c-myc. E RT-qPCR analysis of FBP1 mRNA expression in cells with the silencing of c-myc. F Representative immunofluorescence staining images (×1000) showing that C-MYC inhibited the expression of FBP1 in the cytoplasm (green). Blue dye (DAPI) indicates the nucleus. G Luciferase reporter assay in A2780 and SKOV3 cell lines with c-myc downregulated. H The results of ChIP analysis showed that C-MYC can bind to the FBP1 promoter region. I Luciferase reporter assay of C-MYC mutant sites in the promoter region of FBP1. *P < 0.05, **P < 0.01.

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