Fig. 5: SAMMSON inhibition reduces tumor growth in vivo.

A Relative tumor volume of MEL077 PDX mice (MEL077-2) subcutaneously injected with NTC ASO or ASO 3 (10 mg/kg). Data are mean ± s.e.m. of multiple replicates (n = 4). P values were calculated using two-way ANOVA with Sidak’s multiple testing correction. B Representative tumors of MEL077 PDX mice (MEL077-2) 22 days after treatment (n = 4/treatment group). C Tumor weight of tumors as shown in B. Individual data points and mean are presented. P value was calculated using unpaired two-tailed t-test. D Relative tumor volume of MP46 PDX mice subcutaneously injected with scrambled ASO (NTC) or ASO 3 (10 mg/kg). Data are mean ± s.e.m. of multiple replicates (n = 8–9/treatment group). P values were calculated using two-way ANOVA with Sidak’s multiple testing correction. E Representative images of H&E, Ki67 and Caspase-3 staining of MEL077 tumor sections (MEL077-2) 22 days after treatment with NTC ASO or ASO 3. F Percentage of Ki67 positive cells in 500 counted cells per tumor sample (MEL077-2, n = 4/treatment group) (magnification x100). Individual data points and mean are presented. P value was calculated using unpaired two-tailed t-test. G Number of mitotic cells based on H&E staining (MEL077-2) in E of 10 random high power fields (HPFs) (magnification x400). Each data point represents the mean of 10 HPFs per tumor sample (n = 4/treatment group). P value was calculated using unpaired two-tailed t-test. H Caspase-3 scoring based on morphology and staining intensity as shown in E of four tumor samples per treatment group (MEL077-2). I Selected GSEA results of RNA seq data from UM cell lines OMM1 and 92.1 and UM MEL077 PDX tumors (MEL077-2) demonstrating overlapping enrichment of gene sets involved in apoptosis, p53 and translation upon ASO 3 treatment. The depth of the color represents the normalized enrichment score (NES). The area of the circle represents the false discovery rate (FDR). J Human DNA load measured in lung tissues of MEL077-2 (n = 4/treatment group) and MP46 (n = 10–11/treatment group) mice by means of qPCR of Alu-Sq repetitive sequence. Individual data points and mean are presented. P value was calculated using unpaired one-tailed t-test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.