Fig. 1: Identification of THEMIS2 as the sole common differentially expressed (DE) gene among the sphere-derived CSC-enriched cell lines for TNBC and ovarian cancer. | Oncogene

Fig. 1: Identification of THEMIS2 as the sole common differentially expressed (DE) gene among the sphere-derived CSC-enriched cell lines for TNBC and ovarian cancer.

From: Novel function of THEMIS2 in the enhancement of cancer stemness and chemoresistance by releasing PTP1B from MET

Fig. 1

A Top: Formation of spheres under the stem-cell-selective condition on day 8 after culturing from parental Hs578T, BT549, and MDA-MB-468 cells; bottom: the number of DE genes implicated in Hs578T, BT549, and MDA-MB-468 sphere cells are shown in the Venn diagram. Downregulated and upregulated DE genes (indicated in blue and yellow, respectively) are presented in the bar chart. B Top: Formation of spheres under a similar condition from parental OVS1 and SKOV-I6 lines; bottom: the number of DE genes implicated in OVS1 and SKOV-I6 sphere cells is shown in the Venn diagram. C The DE genes implicated in TNBC and ovarian cancer sphere cells were compared to identify a sole common DE gene as shown in the Venn diagram. D The mRNA expression levels of THEMIS2 in parental MDA-MB-231, MDA-MB-468, Hs578T, BT549, SKOV-I6, and OVS1 cells, as well as in their respective sphere cells, were analyzed using qRT-PCR. E The protein expression levels of THEMIS2 in TNBC sphere cells relative to those of their parental cells were analyzed by immunoblotting. Actin served as an internal control. The histograms represent means ± standard errors (SEs) from three independent experiments (***P < 0.001).

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